A Simple Key For cytometer Unveiled
A Simple Key For cytometer Unveiled
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Data-enriched characterization of mobile populations. Morphological data from photographs can increase to your richness of flow cytometry details. For example, the figure displays an or else standard apoptosis assay utilizing Annexin V and PI, including mobile imaging to characterize cells in Each and every populace to expose morphologically distinct functions. These insights couldn't have been received from multiplex staining by itself.
As samples are acquired within the Attune CytPix Flow Cytometer, the substantial-velocity brightfield digital camera captures and suppliers photos of detected situations in a charge nearly 6,000 illustrations or photos per second, with regards to the flow price and picture sizing. For higher flexibility, Attune Cytometric Application helps you to alter the image capture frequency as desired and select a particular gate for image seize, if demanded.
Combine acoustic concentrating technology with superior-parameter Evaluation for versatile spectral unmixing and conventional workflows.
Find new insights into your experimental knowledge by visualizing populations applying merged fluorescence and morphology knowledge. Our software interface wizards help downsampling, concatenation (merged information) and dimensionality resources which might be created to the software package for an intuitive, streamlined data Examination expertise.
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Below, a highly skilled consumer has gated singlets confidently. Soon after evaluating the manual singlet gate, the CytPix impression-derived parameter ParticleCount reveals this gate has greater than 4% aggregates.
Pair your Attune flow cytometer with an autosampler for elevated workflow effectiveness, and add robotic integration for maximum throughput.
Reliable image high-quality even at higher throughput Acoustic focusing as well as a significant-velocity camera combine to impression these T cells constantly at very low or significant flow rates. Effortlessly alter concentrate and digital camera configurations to satisfy experimental specifications.
Early indications of this contamination experienced originally been dismissed as particles. Backgating to find out new populations. Backgating imaged cells to the Attune CytPix Flow Cytometer also lets you use morphological functions to find exciting subpopulations that will not be apparent from flow cytometry facts by yourself.
End users can look at event photos in true-time to substantiate sample top quality, optimize protocols, and document the morphology of each and every cell inhabitants discovered for analysis. Imaging seize also benefits from acoustic focusing, which really helps to place the cells to make sure that a sharp, centered impression is attained.
AI-driven automated graphic Examination Along with the Attune Cytometric Software package translates party options into unique morphology parameters that may be coupled with normal fluorescence and scatter parameters to reveal hugely reproducible populace improvements and distinctive subsets.
We previously shown the power of imaging Auto-T/Ramos cell interactions. Allow’s examine just the populace of biggest fascination, the double favourable gatherings, to learn more. We could now use prolonged morphological parameters (right here, circularity and skewness of intensity) to even more examine the functions of those populations and refine gating on these activities, growing data robustness.
Built-in dimensionality reduction instruments improved resolution into populations. Fluorescently gated populations back again-gated onto UMAP plot to substantiate anticipated populace resolution in lysed whole human blood. Consumer can see occasions from Every single cluster for visual affirmation of mobile phenotypes.
No clean/no lyse leukocyte Evaluation with imaging. Cells have been acquired from 24-hour-outdated blood by dilution in 1 mM EDTA ( Pro UX/UI enables people to train AI styles for expanded programs. (A) The “Coach Design” characteristic delivers annotation applications and serious-time product coaching precision.
At the center of every Attune Flow Cytometer lies our groundbreaking acoustic concentrating technologies, which utilizes sound waves to meticulously align cells in the flow cell. This check here modern approach surpasses standard hydrodynamic solutions, leading to quicker processing speeds, exceptional facts excellent, and enhanced sample throughput.
The Attune Xenith cytometer is usually a significant-parameter spectral analyzer, providing both equally spectral unmixing and traditional flow capabilities for extensive and multipurpose mobile Evaluation.
Agilent Value Assure guarantees you no less than a decade of instrument use from your date you purchase. If not, We'll credit you with the residual value of the method towards an upgraded model.
In this article, we demonstrate that by gating tactics making use of picture-based quantitative parameters can distinguish interacting cells from coincident occasions to a lot more correctly assess interacting cells.